You are doing an experiment involving antibiotic resistance in your study bacteria. This organism grows well on LB - Miller agar plates. You want to make 50 plates (assuming 25ml/plate) of LB-Miller at 1.5% agar concentration. Twenty-five of these plates will be plain, the other 25 need to contain 50 micrograms/ml of Gentamycin for your resistance experiments. You have a stock solution of Gentamycin at a concentration of 5 mg/ml. How would you go about making your plates

Answers

Answer 1

Answer:

See the answer below

Explanation:

The stock solution of Gentamycin has a concentration of 5 mg/ml while each plate needs to contain 50 micrograms/ml of Gentamycin.

5 mg/ml = 5000 micrograms/ml

There is a need to dilute the stock antibiotic solution in order to arrive at 50 micrograms/ml. Using the dilution principle;

m1v1 = m2v2

5000 x 1 = 50 x v2

v2 = 5000/50 = 1000 ml

Hence, in order to prepare 50 micrograms/ml, 1 ml of the stock Gentamycin should be taken and diluted with 999 ml of distilled sterilized water. 1 ml of the diluted Gentamycin will then be added to each agar plate while they are still in the molten form at a warm temperature.


Related Questions

What is the diference between the question and hypothesis steps in scientific inquiry? a. The question step provides the question to be studied, while the hypothesis step provides a proposed answer to the question b. The question step involves putting the hypothesis into question format C. The question is the problem to be studied, while the hypothesis is the answer found alter experimentation, d. There is effectively no difference between the two steps. Please select the best answer from the choices provided.​

Answers

Answer:

the answer is ( A )

Explanation:

poggers

A meteorologist who has been analyzing weather data from the last fifty years found evidence of climate change. An ecologist has been studying deposits of pollen from the last fifty years to track the types of plants that grew
in certain regions. She has also found evidence of climate change. What does this imply about science?
Meteorology and ecology are the same type of science
When scientific disciplines overlap, researchers should choose new topics
O Different scientific disciplines can be used to investigate similar questions
There should be fewer sciences, as they seem to answer the same questions

Answers

The correct answer is C. Different scientific disciplines can be used to investigate similar questions

Explanation:

The general purpose of science is to study, analyze, and understand the world and especially natural phenomena. Moreover, science includes multiple disciplines with different focuses; for example, astronomy focuses on objects in the space.

Despite this, all disciplines in sciences have objective methods. This causes scientists to obtain the same results or findings even if they belong to different disciplines, and therefore, the same questions or phenomena can be studied from different disciplines. An example of this is that a meteorologist and an ecologist find evidence of climate change. According to this, "different scientific disciplines can be used to investigate similar questions".

Answer:

The correct answer is C. Different scientific disciplines can be used to investigate similar questions

Which is an abiotic factor of a marine ecosystem?
A. food produced
B. temperature change as you go in deeper in the ocean
C. reproduction of animals

Answers

Answer:  The topmost part of a marine ecosystem is the euphotic zone, extending down as far as 200 meters (656 feet) below the surface.

Explanation: Sunlight is one the most important abiotic factors for marine ecosystems.

Hope this helped! :)

In the
part of photosynthesis, sugar is formed.

Answers

Answer:

Light-Dependent reaction

Explanation:

Its logic really, a way for a plant to receive and begin photosynthesis is by sunlight. Therefore when there's a light dependant reaction, sugar is formed. hope it helped

[tex]C_3[/tex] photosynthesis is used by most plants in which a three-carbon compound called 3-phosphoglyceric acid is produced during the Calvin cycle, which becomes a sugar called glucose.

What is [tex]C_3[/tex] photosynthesis?

Light energy capture for sugar production during [tex]C_3[/tex] photosynthesis occurs within the chloroplast where light energy is captured by a noncyclic electron transport process that uses the thylakoid membrane for the necessary electron transport. About 85% of plant species are  [tex]C_3[/tex]plants.

During this pathway, the first molecule produced in the cycle which is a 3-carbon molecule called 3-phosphoglyceric acid, so it is called [tex]C_3[/tex]photosynthesis.

Thus, [tex]C_3[/tex] photosynthesis is used by most plants in which a three-carbon compound called 3-phosphoglyceric acid is produced during the Calvin cycle, which becomes a sugar called glucose.

Learn more about  [tex]C_3[/tex] photosynthesis,  here:

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What is the interpretation of the influence of the heavenly bodies on
human affairs.
O Astronomy
Astrology
Asteroid
Android

Answers

astrology! good luck and i hope you have a good rest of your day!!

What do you think the job of lysosomes is
based on the short animation provided?
hold DNA
build things
digest things
let things in and out of cells

Answers

Answer:

digest things

Explanation:

You can see in the animation that lysosome is digesting

PLS HELP :( 25 POINTS + BRAINLIEST!!

Answers

I believe the answer is (C) Bacteria decomposing dead materials in which returns the nutrients to the soil.

Because the life process of a plant, includes fuel from the sun but also the soil and within the soil their is decomposing materials, as in bacterial substances. From my own knowledge and process of elimination the only option that stands out is (c) sorry but I hope it is right, I really think it is. Good luck!

What does a pyramid of biomass represent ?

Answers

Answer:

A pyramid of biomass shows the total biomass of the organisms involved at each trophic level of an ecosystem. ... There can be lower amounts of biomass at the bottom of the pyramid if the rate of primary production per unit biomass is high.

Explanation:

Answer:

A.

Explanation:

Number of total organisms within each trophic level.

10 things you can do to help save the earth
HELPPPPP!!!!!

which steps can a community take to help its members follow the guidelines? state your answer as a claim. support your claim with evidence from the article. think about how communities can make doing these things easier. then explain why the evidence from the text supports your claim

Answers

Reduce, reuse, and recycle. Cut down on what you throw away. Follow the three "R's" to conserve natural resources and landfill space.

Volunteer. Volunteer for cleanups in your community. You can get involved in protecting your watershed, too.

Educate. When you further your own education, you can help others understand the importance and value of our natural resources.

Conserve water. The less water you use, the less runoff and wastewater that eventually end up in the ocean.

Choose sustainable. Learn how to make smart seafood choices at www.fishwatch.gov.

Shop wisely. Buy less plastic and bring a reusable shopping bag.

Use long-lasting light bulbs. Energy efficient light bulbs reduce greenhouse gas emissions. Also flip the light switch off when you leave the room!

Plant a tree. Trees provide food and oxygen. They help save energy, clean the air, and help combat climate change.

Don't send chemicals into our waterways. Choose non-toxic chemicals in the home and office.

Bike more. Drive less.

Answer:

5 things to save the earth(i can't think of any more)

Explanation:

reuse a water bottle

pick trash up

take shorter showers

recycle

open a window instead of using the AC

How would you go about isolating your pure culture?

Answers

Explanation:

Simpler methods for isolation of a pure culture include: (i) spread plating on solid agar medium with a glass spreader and (ii) streak plating with a loop. The purpose of spread plating and streak plating is to isolate individual bacterial cells (colony-forming units) on a nutrient medium.

a large hurricane destroys a coastal habitat. what type of disruptive event does this describe?

periodic
random
extinctive
episodic

Answers

Answer:

periodic

Explanation:

Answer:

Periodic

Explanation:

What type of electrical charge do atoms have?

Answers

There are 3 types of particles Protons, Neutrons, and Electrons. Now protons and neutrons have a positive charge while electrons have a negative charge.

Mutations in several different genes that encode enzymes in the pathway for the synthesis of the heme group may cause a genetic disorder called methemoglobinemia. The symptoms of this disorder range form mild to death in the most severe cases. The enzyme cytochrome b5 reductase is one of the enzymes in this pathway. This enzyme is responsible for reducing the iron in the heme group to the ferrous state so that it can bind oxygen. Kobayashi et al. reviewed several mutations in this gene. One of these mutations causes a Ser to Pro substitution. Explain what nucleotide change and in which position in the codon can cause this amino acid change. Assign all possible appropriate terms to this mutation.

Answers

Answer:

Please find the explanation of the nucleotide change below

Explanation:

Mutation is any change that occurs to the nucleotide sequence of a gene. According to this question, a substitution mutation which causes the Serine amino acid to change to Proline amino acid in the cytochrome b5 reductase enzyme is described.

A substitution mutation is a type of mutation in which one or more nucleotide base is replaced by another. The mRNA codons that specify Serine and Proline amino acids are as follows:

Serine: UCU, UCC, UCA, UCG, AGU, AGC

Proline: CCU, CCC, CCA, CCG

Based on the observation of these bases, URACIL BASE in the first four listed codons for serine amino acid is replaced by CYTOSINE BASE. i.e.

UCU (serine) -> CCU (proline)

UCC (serine) -> CCC (proline)

UCA (serine) -> CCA (proline)

UCG (serine) -> CCG (proline)

Notice that these base substitutions (U-C) occur in the 1st position of the codon.

How are Metamorphic Rocks formed?

A. through compaction and cementation

B. through crystallization and solidification

C. through heat and pressure

D. through weathering and erosion

Answers

C. Through heat and pressure
Hope this helps!

A manager wanted to test whether the amount of noise in a clothing factory affected how
much work employees accomplished. Three groups of employees were tested every day
over a 5-day period. All three groups did the same type of work, they were all equally trained
to do the work, they used the same equipment, and they were tested the same time of day.
Group one was given headphones that blocked out all of the outside noise. Group two was
given headphones that blocked out some of the noise. Group three was not given anything
to block out the noise. The number of shirts made by the employees in one hour was
determined.
What is the independent/manipulated variable?
the amoutn of noise
the number of shirts made by the employee
the type of music the employees listen to while they work.
The amount of training employees need.

Answers

Answer:

When seeking to determine the number of shirts made by employees subjected to noise with and without the use of headphones, the independent variable is the amount of noise.

Explanation:

During an investigation, the independent variable may be subject to change, in order to observe the effect it will have on the dependent variable.

In the case presented, the amount of noise to which employees are exposed represents the independent variable, which is modified by the use of headphones that block all or part of the noise.

The productivity —expressed in the number of shirts made— can be expected to be higher for employees not exposed to noise.

Other options do not apply because:

The number of shirts made by the employee. Dependent variable .The type of music the employees listen to while they work or  the amount of training employees need. These are not the variables studied.

this compound makes up the building blocks for all plant structure. plants can use it for energy and animals eat the plant to be able to get energy from this organic which organic compound is this?

Answers

The answer is :-

protein

Which examples describe results of globalization?



Choose all answers that are correct.


spread of innovations


increase in death rate


damage to some cultures


cultural blending

Answers

Answer:

b

Explanation:

A characteristic which differentiates eukaryotic cells from
prokaryotic cells is eukaryotic cells have-
A. a cell membrane that controls the flow of materials in and
out of the cell
B. genetic material (DNA) which controls the cell's activities
C. a jelly-like substance called cytoplasm that holds the
organelles
D. a centrally located nucleus that contains the DNA of the
cell

Answers

Answer:D.

Explanation: Eukaryotic cells have a nucleus while prokaryotic cells dont. The nucleus store the DNA so that means that prokaryotic cells have their DNA floating around in them while eukaryotic cells have their dn6in the nucleus alone.

Answer:

D

Explanation:

got an 100 on edge :)

The genetic code is ____________because almost every living organism has the same genetic code.

Answers

The genetic code is *UNIVERSAL*

How is osmosis different from diffusion?

A. Osmosis refers to substances moving up a concentration gradient, while diffusion refers to substances moving down a concentration gradient

B. Osmosis is a form of passive transport, while diffusion is a form of active transport

C. Osmosis is a form of active transport, while diffusion is a form of passive transport,

D. Osmosis refers specifically to the diffusion of water.

Answers

I’m pretty sure it’s A but not positive

Help me plz hurrrrrrrrrrrrry An ice cube is placed inside a thermos and the lid is closed. The ice cube
eventually melts. Most of the thermal energy that melts the ice comes from
which source?
O A. The liquid water that forms
OB. The air surrounding the thermos
O c. Water vapor from the air
O D. The bonds between water molecules

Answers

i think it might be b but i’m not 100% sure

Answer:

its B

Explanation:

coz it is

Write a sentence or two connecting these terms highlighting each: Genes, genetics, Genome, protein, trait.

Answers

Answer:

Please find the sentence below

Explanation:

GENETICS is the study of individual genes present in the GENOME of an organism. In the GENOME, which is the complete set of GENES in an organism, genes that contain the information needed for the synthesis and transmission of useful products called PROTEINS undergo genetic expression. Gene expression involves two major processes viz: transcription and translation.

Transcription involves the synthesis of an mRNA from the information encoded by a GENE while translation involves the synthesis of a PROTEIN from the information encoded by the mRNA transcript. The produced protein is responsible for bringing about certain TRAITS, which are observable characteristics, in living organisms.

Therefore, the central dogma of biology is the process that sees GENE converted to mRNA and mRNA converted to PROTEIN.

Can someone please try and help me? I've been getting ignored so much, this is due at 4:50. Please please, help :( answer all

Answers

Answer:

1. Radiant Energy

number 13 is Trade Winds

The diameter of a human blood cell is 0.0000062 m. How can this number
best be expressed in scientific notation?
A. 6.2 x 10-5 m
B. 6.2 x 10-6 m
C. 6.2% -106 m
D. 62 x 10-5 m
SUBMIT

Answers

Answer: B. 6.2 x 10-6 m

Explanation:

just took the test and it is B.

Scientific notations are used to express numbers that are too large of too small. The diameter of human blood cell is 0.0000062m can be written as 6.2 × 10⁻⁶m.

Thus, the correct option is B.

What is Scientific notation?

Scientific notation is a way of expressing numbers that are too large or too small to be conveniently written in decimal form. It may be referred to as scientific form, or standard form in the United Kingdom.

Scientific notations can be written with the base of the number be always 10. The exponent must be a non-zero integer, it can be either positive or negative. The absolute value of the coefficient is greater than or equal to 1. Coefficients can be positive or negative numbers including whole and decimal numbers depending upon the value of the number. For example, 650,000,000 can be written in scientific notation as 6.5 × 10⁸.

The diameter of human blood cells = 0.0000062 m

It can be written as 6.2 × 10⁻⁶m as well for easy understanding.

Therefore, the correct option is B.

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Thuy wants to advocate for clean air laws. Which field of study would be most useful to her?
A. Zoology
B. Microbiology
C. Earth science
D. Marine science

Answers

Answer: C. Earth Science

Explanation:

Answer:

c.

Explanation:

just took the test

Imagine a bacterial population of 100 cells. Five of the cells are resistant to sulfonamides, and six other cells are resistant to erythromycin. In the development of drug-resistant bacteria, which of the following selects for resistant bacteria? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all.

Answers

Answer:

39

Explanation:

When u have 100 cells and most of them are resestant to drugs most people have 39 cells

When  we have 100 cells and most of them are resestant to drugs most people have 39 cells.

Thus, Bacterial communities invariably include a variety of clonal lineages. Since recombination exchanges across lineages and species mobilize tiny genomic fragments, bacterial populations are not fully asexual.

Recombination, as opposed to de novo mutation, contributes differently to the creation of novel bacterial genotypes in different bacterial populations, and as this contribution rises, clonality in a particular population diminishes.

As a result, there is a wide range of potential population architectures, with only a few bacterial species existing at the extremes of being entirely non-clonal and very clonal. the examination of samples of bacterial isolates that are true representations of the natural population.

Thus, When  we have 100 cells and most of them are resestant to drugs most people have 39 cells.

Learn more about Bacterial population, refer to the link:

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Scientists connect data points on graphs with a dashed line because information between the plotted data is:

approximate
inferred
interpolation
all of the above

Answers

The answer is all of the above

Answer:

all of the above

i just took the lesson

Why will an Elodea plant from a freshwater pond wilt when placed in salt water?
A. The vacuole will begin to expand because of extreme pressure.
B. The cell wall will begin to expand because of extreme pressure.
C. Freshwater will move out of the cells of the plant.
D. Salt water will move out of the cells of the plant.

Answers

Answer the answer is  (c)

A strange new species of plant has been discovered. When studying the chromatin from the cells of this plant using the kinds of experiments described above in (b) a 120-bp fragment of DNA is seen. Analysis of the histone core reveals histones in the following proportions: H2A 33.3% H2B 33.3% H3 0% [no histone H3 found] H4 33.3% On the basis of these observations, what conclusions can you make about the probable structure of the nucleosome in the chromatin of this plant? Be specific in describing the nature of the nucleosome: which histones form the core (1pt), how many of each are in there (1pt) and how much core-DNA is around it (1pt).

Answers

Answer:

The nucleosome core is composed of two each of histones H2A and H2B and H4

Explanation:

The nucleosome is the basic structural unit of all eukaryotic genomes. A nucleosome is composed of approximately 146 base pairs of DNA sequence wrapped around a core of histone proteins, which generally consists of two subunits of the histones H2A, H2b, H3 and H4, respectively (i.e., an octamer of histone proteins). Within the cell nucleus, the large DNA molecule is coiled into nucleosomes, this structure is further compacted into chromatin fibers and subsequently into higher-order organization structures to finally form a chromosome.

How does water move on Earths surface?

Answers

Answer : Water moving across the earth in streams and rivers pushes along soil and breaks down pieces of rock in a process called erosion. The moving water carries away rock and soil from some areas and deposits them in other areas, creating new landforms or changing the course of a stream or river.

Hope this helps! good luck!
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