Compound light microscopes and transmission/transmitted light microscopes produce an image based on light transmission through the sample. Explain how the fluorescence microscope layout differs from that of transmission microscopes. Include the source of the light visualized in fluorescence microscopy in your answer.

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Answer 1

The fluorescence microscope differs from the compound light, and transmitted light, not so much because of the structure, but instead because of how light is used to generate an image/ see the sample.

In the fluorescence microscope, the light does not go through the sample per se, instead, a filter is needed, it works with a higher wavelength, in this case, the light rays go to the sample and excite the species marked with the fluorescence substance, that is to say, the sample must be previously treated so it can absorb light and re-emitted as fluorescence (electromagnetic radiation) therefore creating an image, of light. This is possible due to the proprieties of some molecules, such as fluorochromes.


Related Questions

how is the rate of photosynthesis affected when leaf disks are exposed to different concentrations of bicarbonate solution?

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The rate of photosynthesis affected when leaf disks are exposed to different concentrations of bicarbonate solution when the leaf disks immersed in the solution with the highest proportion (0.8% sodium bicarbonate solutions) rose in an average time of 4.91 minutes. This experiment demonstrates that the rate of photosynthesis can increase when there is more carbon dioxide present.

Photosynthesis- The method through which certain living animals and green plants convert sunlight into chemical energy. In green plants, visible light is gathered and utilized during photosynthesis to transform water, carbon dioxide, plus carbohydrates to make the glucose molecule. The photosynthesis occurs in two ways either PSI or PSII.

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What’s the correct answer answer asap for brainlist

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Answer: B

Explanation:

Vascular plants have several specialized organs. Which one is matched correctly?A. Fruits: specialized in reproductionB. Cones: Specialized in reproductionC. Flowers: Specialized in reproductionD. All of them are correct

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The vascular plants have several specialized reprodutive organs, which one responsible for one stage of the process of reproduction. The flowers are reprodutive organs, as well as fruits and cones, however they function in reproduction is distinct. The flowers is the most complex reproductive organ, since it is composed by for different structures (carpels, staments, petals and sepals), the cones are where the seeds are produced in the female plants during sexual reproduction after being fertilised by male gametes (pollen), and the fruits are the result following the pollination that are important to continuity of plants generations. Therefore, the alternative D (all of them are correct) is the answer for that question.

This table suggests that: A. a pH of 9 is the optimal pH for all enzymatic reactions. B. enzymes have particular pH's at which they function best. C. reactions proceed at the same rate for all enzymes. D. as the pH increases, the reaction rate always increases.

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The answer to this question is B. enzyme have particular pH's at which they function best, since we can see both enzymes at different pH values, have different reactions rates: considering enzyme 1, the highest reaction rate is in pH 10, and considering the enzyme 2, the highest reaction rate is in pH 9.

The F1 offspring of a cross has been provided for you. It is your job to work backwards in order to determine the genotypes, the genetic makeup, of BOTH parents that produced each cross. More specifically, what parental genotypes would give you these possible genotype combination?

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To fill the Punnett Square we must place the alleles from one of the parents horizontally oriented on the top of a 3x3 diagram, and the alleles from the other parent vertically oriented on the left border of the diagram. After that, we must cross each one of the alleles from one parent with the alleles of the other, positioning the result of those crossings (Ee) in the blank space where those alleles meet. Therefore, we must look at the combinations and extract the allele that appears in the same line or in the same column, as follows:

Therefore, the parental genotypes are: EE x ee.

Each offspring must have 1 copy of each


chromosome, either S or s, T or t.


What possible combinations could form from this offspring?

Answers

The possible combinations form from an offspring in which parents have  ST and st genotypes is SSTT, SSTt, SStt, SsTT, SsTt, ssTT, ssTt and sstt, it is supposing that S and T genes are not linked.

How genes are inherited in offspring?

In offspring, genes are inherited as independent units in the case that genes are not located on the same chromosomes. This law is known as the principle of independent segregation.

In this case, parents may form all types of gamete combinations and therefore offspring may exhibit any combination.

Therefore, with this data, we can see that parents in this case may form different gamete combinations for S and T genes that will be inherited in offspring.

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a mosquito repellent is sprayed on one arm and the other arm is not sprayed. The repellent is an example of in independent or a controlled variable.

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A mosquito repellent which is sprayed on one arm and the other arm is not sprayed, is an example of independent variable.

A mosquito repellant is a chemical substance applied on the skin, clothes or any non-living object to avoid the appearance of mosquitoes on that surface. It is made of constituents like DEET, metofluthrin, oil of lemon-eucalyptus, picaridin, etc.

Independent variable in an experiment is that parameter which is changed. It has the potential to alter the results of the experiment as the effect on the experiment are influenced by this variable. It is called independent because no other variable can make changes in its effect.

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explain why something is biodegradable

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If products do not decompose, ii will commonly dwell in landfills and so much area will be taken up. Biodegradable means that there is the abililty to be decomposed and there is no harm in the ecosystem produced by this process. Materials or objects can be broken down to smaller parts and absorbed into the soil that will serve as nutrients.

Which sentence describes an example of how a living thing might grow and
develop?
A. A worm moves away from light.
B. A single cell divides into multiple specialized cells.
C. A rock breaks into multiple pieces as it rolls downhill.
D. A fire spreads through a forest.

Answers

Answer: B. A single cell divides into multiple specialized cells.

Explanation:

B quite literally explains what happens to our bodies, we start with stem cells that divide and become specialized to produce all the different types of cells we need to grow and develop.

B A single cell divides into multiple specialised cells

Which of the following substances is not an element?Iron, oxygen, water, carbon,hydrogen

Answers

The substance that is not an element is water, it is formed by one atom of the element oxygen and two atoms of the element hydrogen.

The rest of the substances, iron, oxygen, carbon and hydrogen, are pure elements formed by equal atoms.

Draw and label a simple diagram to show how DNA is constructed from sugars, phosphates and bases.

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The DNA is constructed from sugars, phosphates and bases as follows:

What are three types of freshwater wetlands?

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Freshwater wetlands are ecosystem affected by inundation (permanently or temporary). The three major types are swamps (uncultivated low-lying ground where water collects), marshes (low-lying and waterlogged land, flooded in wet seasons or at high tide) and bogs (wet muddy ground that's too soft to support a heavy body).

Which group creates the most pollution?O developing countriesO hunter-gatherersO developed countriesO special interest groups

Answers

What creates most pollution is the burning of fossil fuels to produce energy for eletricity and transportation, because of the release of carbon dio

How did the Asian carp get to America and why are they a problem? Answer the question completely. Support your arguments with quotes from the text. Explain the how your quotes support your arguments.

Answers

Answer:

Asian carp were brought into the United States intentionally by humans to capitalize on the carps dietary preferences.

Explanation:

When an animal is hungry it hunts for food. In this case, what is the stimulus?A. hungerB. hunting

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And stimulus is a change in the organism or the environment that triggers a reaction, in this example, hunger causes the animal to hunt, so hunger would be the stimulus.

Hi I’m not sure if I’m right with this Question I need some help please and thank u

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The reason why these cells can develop too many tasks at once, beyond having the specific genes expressed, is that they can constantly produce usable forms of energy in form of ATP by using glucose in cellular respiration. So the correct answer to this question is the final one.

Which statement describes the offspring cells produced during this process?

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Mitosis produce 2 identical daughter cells. Each of these cells have the same number of chromosomes as the parent cell.

The budding yeast cell that is undergoing mitosis have the same number of chromosomes as the parent cell. Meiosis produce daughter cells having half the number of chromosomes of the parent cells.

Answer - Option 2 - They have the same number of chromosomes as the parent cell

Available energy ___________ as it is transferred from one organism to another in a food chain.

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Answer:Key Points. Energy decreases as it moves up trophic levels because energy is lost as metabolic heat when the organisms from one trophic level are consumed by organisms from the next level.

Explanation:

If your digestive system is damaged or fails, how are other body systems or the body as a whole affected?

Answers

Body systems must work together. If one system will be damaged or fails, the other systems will be affected. If the digestive system will be fail, digestion will fails. Water will not be absorb that will lead to the circulatory system to have difficulty in the consistency of blood. If there is a presence of digestive disease, there will be an interference in the digestion as well. Other organ functions will be affected, too. Several effects will be seen such as dehydration, poor absorption of nutrients, blocked blood flow, etc. If the digestive shuts down due to damage of muscles or nerves, it may cause nausea, vomiting, lack of appetite and malnutrition. The nervous and circulatory systems work with the digestive system and these systems are greatly affected if it will not work properly.

The respiratory system is affected if the digestive system fails. These two systems work together to provide power to the entire body system. If digestion fail due to overeating, it can cause abdominal bloating. Bloating can lead to shortness of breath.

Which of the following are formed during photosynthesis?A. C6H1206 and 0₂OB. C₂H4O2 and 02OC. C6H1206 and H₂0OD. C₂H4O2 and H₂O

Answers

A. C₆H₁₂O₆ and O₂ are formed during photosynthesis.

6CO₂+6H₂O→C₆H₁₂O₆+6O₂; Photosynthesis' Reaction

Photosynthesis is a prominent reaction in green plants. Chlorophyll is the main pigment responsible for trapping sunlight. Stomata primarily capture carbon dioxide, one of the raw materials. Water and oxygen are the by-products of it.

Glucose is the primary product formed in the process. The photosynthetic products are transported as sucrose and stored as starch.

Photosynthesis is divided into Light reaction and Dark reaction, on the basis of the availability of light. Carbon dioxide is reduced to carbohydrates and water is oxidized to oxygen.

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Explain why it is that we have periods of night and periods of day?

Answers

The Earth orbits the sun once every 365 days and rotates about its axis once every 24 hours. Day and night are due to the Earth rotating on its axis, not its orbiting around the sun. The term 'one day' is determined by the time the Earth takes to rotate once on its axis and includes both day time and night time.


Which phrase describes a density-independent limiting factor that can affect
Fiting
ecosystem stability?
A. High herbivore emigration from a grassland
B. Dune erosion by ocean water along a shoreline
C. An increase in the carnivore population in a desert
D. Two large populations competing for the same food source

Answers

The phrase dune erosion by ocean water along a shoreline best describes a density-independent limiting factor that can affect ecosystem stability (Option B).

What is a density-independent limiting factor?

A density-independent limiting factor can be defined as any factor in a given ecosystem that may alter the homeostasis of the population that lives in a given geographic area.

These factors (density-independent limiting factors) are generally abiotic factors such as hurricanes, extreme temperature conditions, the presence of contaminants in the air that hamper life in a given area, etc.

Conversely, density-dependent limiting factors are biotic factors such as competitive species that alter the development of another population.

Therefore, with this data, we can see that a density-independent limiting factor is any abiotic condition that may alter the life of a population in a give geographic area and thus alter the homeostasis of the whole ecosystem.

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Answer:D

Explanation:

Which state of matter does this model represent?

Answers

The state of matter that this model represents is Liquid. Thus, the correct option is B.

A disadvantage of asexual reproduction is that offspring _______. A. may not be as able to respond to and survive changes B. have a greater chance of getting harmful genes C. receive more variability in genes from a single parent D. inherit a mix of two parent’s genetic information

Answers

Answer:

the offspring are genetic clones and there is lack of genetic variation among the offspring.

Explanation:

4 things yk about matter (in science) WILL GIVE BRANILIEST!!

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-Some physical characteristics of matter are shape, color, size, and temperature
-Matter can be solid, liquid, or gas
-Matter makes up everything around us
-Anything that occupies space and has mass is called matter.

All animals area. polytrophsb. heterotrophsc. autotrophsd. biotrophs

Answers

Animals

All animals are heterotrophs (option B), which means that they cannot produce their own food, and therefore they need to feed on different types of organic matter in order to obtain energy for survival.

You are examining a patient who has been in an accident. You test their motor skills and find that they can move their limbs but cannot write with a pencil and stumble often when walking. You conclude that they have damage to the part of the brain that governs fine muscle control. What area is this?A. Brain stemB. PituitaryC. Cerebral cortexD. Cerebellum

Answers

The answer is the letter D. Cerebellum- responsible for fine muscle control.

*Cerebellum is responsible for maintaining balance and posture.

How can photochemical smog impact the environment? It can increase the number of insects in the area. It can reduce the amount of plastic in the ocean. It can increase tree and plant growth in a city. It can decrease the crop yield in a region.

Answers

The Photochemical smog impact the environment by decrease the crop yield in a region.

Photochemical Smog- When nitrogen oxides & organic compounds (VOCs) combine with sunlight, a mixture of pollutants called photochemical smog is produced, which explains why there is a brown cloud above cities. Due to the fact that we receive the most sunshine in the summer, it tends to happen more frequently. primary contaminates.

Global warming is being caused by the massive amount of fossil fuels which being consumed, which is increasing the amount of acid rain & photochemical smog produced as well as the quantity of atmospheric carbon dioxide.

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elect 2 that apply.Which of these are monotremes? Select all that apply.kangaroosgiraffeduck-billed platypusdogkoala bearsspiny anteater

Answers

Answer:

Duck-billed platypus and spiny anteater

Which of the following biological molecules are composed of the elements C, H, O only

Answers

The biological molecule composed of elements C, H, O only is:  (2) carbohydrate.

Biological molecules are the organic polymers present inside the body that help in the proper maintenance and functioning of the body. The major biological molecules present inside the body are: carbohydrates, proteins, nucleic acids and lipids.

Carbohydrate is a polyhydroxy ketone or aldehyde. They are of three types: monosaccharides, disaccharides and polysaccharides. Mono saccharides are the basic monomeric unit that form the di- and poly-saccharides. They serve several functions like structural, energy, food, etc. Examples are glucose, starch, glycogen, etc. It is a very essential nutrient in foods.

The question is incomplete, the complete question is:

Which of the following biological molecules are composed of the elements C, H, O only?

nucleic acidcarbohydrateproteinlipid

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